The Isolation and Structural Determination of Flavonoids from Justicia Gendarussa

The current study was intended for isolation of bioactive polyphenolic compounds from methanol extract of Justicia Gendarussa and their subsequent characterization. The flavonoid compounds were isolated and identified by using thin layer chromatography (TLC), were qualified by using reverse phase high performance chromatography (RP-HPLC). Their structures and chemical bonds were analyzed using Ultraviolet-Visible spectrophotometer (UV-VIS spectrophotometer), Fourier Transform-Infra Red spectroscopy (FTIR), Nuclear magnetic resonance NMR (13C and 1H),LC-MS, DSC, SEM and EDX techniques. Two flavonoids were identified as Apigenin and Vitexin. For the first time Apigenin and Vitexin have been isolated successfully from the Justicia Gendarussa. The isolation of the above considered flavonoids would be helpful to practise plant-based pharmaceutical research to treat various difficulties linked with human diseases.